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KMID : 0613820140240050476
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Journal of Life Science
2014 Volume.24 No. 5 p.476 ~ p.484
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Inhibitory Effects of Lyophilized Dropwort Vinegar Powder on Adipocyte Differentiation and Inflammation
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Park Yun-Hee
Choi Jun-Hyeok
Whang Key
Lee Syng-Ook
Yang Seun-Ah
Yu Me-Hee
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Abstract
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Obesity, which is characterized by a state of mild chronic inflammation, is known to cause metabolic diseases. This study was carried out to investigate the effect of lyophilized dropwort vinegar powder (DVP) on adipocyte differentiation and inflammation in T3-L1 preadipocyte and RAW 264.7 macrophage cell lines. DVP inhibited the differentiation of 3T3-L1 preadipocytes induced by a mixture of IBMX, dexamethasone, and insulin (MDI). Western blot analysis of cell lysates showed that DVP decreased the levels of two major transcription factors involved in adipogenesis, peroxisome proliferator-activated receptor-γ (PPAR-γ) and CCAAT-enhancer-binding protein α (C/EBPα). DVP also significantly suppressed lipopolysaccharide (LPS)-induced production of nitric oxide (NO), and this was accompanied by a decrease in inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression. These results demonstrate that DVP inhibits MDI-induced adipocyte differentiation of 3T3-L1 cells and LPS-induced inflammation in RAW 264.7 macrophage cells. The findings indicate that this natural product may be a good candidate as to prevent metabolic diseases.
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KEYWORD
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3T3-L1 adipocyte, anti-inflammatory, anti-obesity, dropwort vinegar, RAW 264.7 macrophages
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